dkk3 treatment  (MedChemExpress)

Journal: American Journal of Translational Research

Article Title: Use of tumor suppressor genes of naked mole rats for human cancer treatment

doi:

Figure Lengend Snippet: Phylogenetic gain/loss trees of DKK3 gene (A) and PDCD5 gene (B) were created in www.ensembl.com. The trees showed the number of DKK3 and PDCD5 paralogues/copies for each species or taxon. Red lines mark significant expansion of the DKK3 and PDCD5 gene copies. Comparison of protein sequence and 3D structure of DKK3 (C) and PDCD5 (D) using the visualization software PyMOL.

Article Snippet: Recombinant DKK3 protein treatment Cells were treated with recombinant human DKK3 (hDKK3, 200 ng/ml), rat DKK3 (rDKK3, 200 ng/ml) or mouse DKK3 (mDKK3, 200 ng/ml) (Creative BioMart, Shirley, NY) for 24 hours.

Techniques: Comparison, Sequencing, Software

Journal: American Journal of Translational Research

Article Title: Use of tumor suppressor genes of naked mole rats for human cancer treatment

doi:

Figure Lengend Snippet: Anti-tumor effect and molecular mechanisms of DKK3 on breast cancer cells. A. Proliferation of human, rat, and mouse breast cancer cells with DKK3 treatment was measured using the MTT assay. B. Changes in β-catenin, p-β-catenin, p-AKT and p-mTOR in DKK3-treated breast cancer cells and their parental cells were studied using western blotting. C. Stemness regulatory transcription factors, SOX2, Nanog, C-myc and Oct4, in DKK3-treated breast cancer cells and their parental cells were studied using RNA sequencing.

Article Snippet: Recombinant DKK3 protein treatment Cells were treated with recombinant human DKK3 (hDKK3, 200 ng/ml), rat DKK3 (rDKK3, 200 ng/ml) or mouse DKK3 (mDKK3, 200 ng/ml) (Creative BioMart, Shirley, NY) for 24 hours.

Techniques: MTT Assay, Western Blot, RNA Sequencing

Journal: American Journal of Translational Research

Article Title: Use of tumor suppressor genes of naked mole rats for human cancer treatment

doi:

Figure Lengend Snippet: Anti-tumor effect and molecular mechanisms of PDCD5 on breast cancer cells. A. Proliferation of human, rat, and mouse breast cancer cells with DKK3 treatment was measured using the MTT assay. B. Changes of β-catenin, p-β-catenin, p-AKT, and p-mTOR in PDCD5-treated breast cancer cells and their parental cells were studied using western blotting. C. Stemness regulatory transcription factors, SOX2, Nanog, C-myc and Oct4, in PDCD5-treated breast cancer cells and their parental cells were studied using RNA sequencing.

Article Snippet: Recombinant DKK3 protein treatment Cells were treated with recombinant human DKK3 (hDKK3, 200 ng/ml), rat DKK3 (rDKK3, 200 ng/ml) or mouse DKK3 (mDKK3, 200 ng/ml) (Creative BioMart, Shirley, NY) for 24 hours.

Techniques: MTT Assay, Western Blot, RNA Sequencing